Fig. 2.

Conditions decreasing intracellular ATP are also associated with decrease in SUR2A and cellular resistance to metabolic stress. Bar graphs depicting ATP (A, D, F) and SUR2A (B, E, G) levels in H9c2 cells treated for 24 h with 50 mM 2-deoxyglucose (A, B, C) or DNFB at concentration of 10 μM (D, E) or 20 μM (F, G) as well as cell survival/death of these cells in response to chemical hypoxia induced by 4 mM DNP (C). Inset in B represent original Western blots for SUR2A under labeled conditions. Each bar is a mean ± SEM (n = 3–5). *P < 0.05. AU = arbitrary units. Western blots and methods used to measure ATP and CK are described in details in refs. [2] and [3].