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Dimensions of the intercalated disc. Myocyte width (A) and intercalated disc area (B) significantly increased between age groups (n = 30; ANOVA p < 0.001). Intensity of total endogenous Cx43 protein from each intercalated disc expressed as a percentage of the total disc area (C) was found to decline with age (n = 50; ANOVA p < 0.0001; linear regression, y = −0.94x + 55.4).
