FIG. 3.
Exclusion of DHBV-ClaI in PDHs. PDHs from congenitally DHBV-infected or uninfected ducklings were harvested and plated as described in Materials and Methods. (A) PDHs from a congenitally DHBV-infected duckling were either mock infected (lanes 1 to 6) or infected with DHBV-ClaI (lanes 7 to 18) at 3 days postplating. Intracellular viral DNA was harvested and analyzed for the presence of DHBV-ClaI. Each lane represents intracellular viral DNA harvested from cells in one well of a six-well culture plate. (B) PDHs from an uninfected duckling were infected with DHBV and DHBV-ClaI simultaneously at 3 days postplating (lanes 1 to 6), infected with DHBV-ClaI alone at 8 days postplating (lanes 7 to 12), or infected with DHBV at 3 days postplating and then infected with DHBV-ClaI at 8 days postplating (lanes 13 to 18). Intracellular viral DNA was harvested and analyzed for the presence of DHBV-ClaI. wt, wild type.