Table 1.
Manipulation | Measurement | All Trials
|
|
---|---|---|---|
WT | Duper | ||
Novel confinement wheels in DD | |||
CT 0 | Phase shift (n) | 0.2 ± 0.4 (15) | 0.04 ± 0.6 (21) |
CT 4 | Phase shift (n) | 0.1 ± 0.4 (18) | 0.3 ± 0.4 (19)* |
CT 8 | Phase shift (n) | 0.03 ± 0.3 (15) | 0.3 ± 0.6 (24) |
Revolutions (all confinements) | 2551 ± 211(48)* | 2583 ± 209 (64)* | |
Control (DD) | |||
CT 4 | Phase shift (n) | −0.03 ± 0.08 (5) | −0.003 ± 0.10 (8) |
Revolutions | 5 ± 2 | 10 ± 5 | |
Light pulses | |||
CT 18.5 | Phase shift (n) | 2.6 ± 0.9 (15)* | −11.6 ± 2.6 (16)*† |
CT 15 | Phase shift (n) | −1.3 ± 1.1 (15) | −5.6 ± 1.1 (16)† |
Modified Aschoff type II novel wheel confinements at ZT 6 | |||
LD to DD | Phase shift (n) | 0.54 ± 0.25 (9) | 1.87 ± 0.98 (6)† |
Revolutions | 522 ± 123 | 1047 ± 364 | |
LD to DD Control | Phase shift (n) | 0.58 ± 0.40 (9) | 2.07 ± 0.83 (6)† |
Revolutions | 3 ± 2 | 183 ± 76* | |
LL to DD | Phase shift (n) | 1.95 ± 0.78 (9)* | See text. |
Revolutions | 733 ± 165 | See text. |
All phase shifts are presented as circular mean ± circular SD given in circadian hours (number of animals). Number of revolutions for all experiments is provided as mean ± SEM. Significant differences (p < 0.05) between experimental and control groups within genotype and between genotypes are indicated (* and †, respectively). Where values are not provided for dupers in the modified Aschoff type II novel wheel confinement experiment, the sample size is too small for statistical analysis; see text for details. WT, wild type.