Skip to main content
. 2015 Jun 3;9:2867–2881. doi: 10.2147/DDDT.S81157

Table S1.

Primers used for RT-PCR assay

Gene ID Gene symbol Sequence (5′–3′) Size of PCR products (bp) Amplification efficiency
24881 Wnt1 Forward primer: CTTCGGCAAGATCGTCAACC
Reverse primer: GCGAAGATGAACGCTGTTTCT
51 101.05
84353 β-catenin (Ctnnb1) Forward primer: TGAAGGTGCTGTCTGTCTGCTC
Reverse primer: TGCATCGGACAAGTTTCTCAGA
143 100.8
84382 TCF4 Forward primer: CCAGTGGAATGAATCAGCCC
Reverse primer: GAGGTGCTGTAATGGTTTGTGC
190 98.9
84027 GSK-3β Forward primer: ATCTTTGGAGCCACCGATTACA
Reverse primer: TTTGCTCCCTTGTTGGTGTTC
160 98.6
293897 Dkk1 Forward primer: CGAGGAAGGCATCATTGAAAAC
Reverse primer: CTGTGGCGCAGTCTGATGATC
143 100.3
25661 Fn1 Forward primer: TGACAACTGCCGTAGACCTGG
Reverse primer: TACTGGTTGTAGGTGTGGCCG
72 99.7
81822 β-actin (Actb) Forward primer: GGGAAATCGTGCGTGACATT
Reverse primer: GCGGCAGTGGCCATCTC
76 105.7

Abbreviations: RT-PCR, reverse transcriptase-polymerase chain reaction; TCF4, transcription factor 4; GSK-3β, glycogen synthase kinase-3β; Dkk1, dickkopf 1; Fn1, fibrone ctin 1.