Table 2.

Average optical density values of protein expressions by immunohistochemistry staining in renal tissues of rats in different groups, $\overline{x}+s(\mathrm{n}=3)$

Group	Masson (%)∇	Wnt1	β-catenin	GSK-3β	Dkk1	TCF4	Fn1
Sham	5.1±0.4	3.31±0.69	1.05±0.08	4.0±0.7	0.42±0.06	2.5±0.1	2.60±0.44
BC	5.3±0.3	3.37±0.04	1.45±0.83	2.7±0.4	0.35±0.06	2.2±0.1	2.90±0.55
5/6 Nx	15.8±1.4#	8.92±0.88#	4.99±1.00#	8.9±1.1#	4.70±0.12#	11.2±1.2#	6.71±0.38#
Losartan	10.9±1.7#,*	8.76±1.54#	3.50±0.35#,*	8.3±1.3#	4.29±0.70	6.7±0.3#,*	5.26±0.11#,*
UCG	9.9±0.8#,*	6.70±0.41#,*	2.54±0.62#,*	7.0±1.4#,*	3.69±0.68	4.3±0.3#,*	5.36±0.17#,*
HGF-L	8.1±0.5#,*,Δ	6.34±0.24#,*,Δ	2.97±0.82#,*	6.3±0.5#,*	3.78±1.15	6.7±0.5#,*	5.56±0.37#,*
HGF-M	7.3±0.9#,*,Δ	5.95±1.11#,*,Δ	2.49±0.57#,*	7.0±1.4#,*	4.96±1.46	6.4±0.5#,*	4.72±0.70#,*
HGF-H	5.6±1.2*,Δ	4.32±0.22*,Δ	1.83±0.16*,Δ	8.8±0.4#	6.62±1.03	6.0±0.5#,*	4.16±0.49#,*,Δ

Notes: All data are showed after multiplying by 10². Compared with Sham and BC groups,

^#P<0.05; compared with the 5/6 Nx group,

^*P<0.05; and compared with the losartan group,

^ΔP<0.05.

^∇the proportion of positive area.

Abbreviations: GSK, glycogen synthase kinase; Dkk1, dickkopf 1; TCF4, transcription factor 4; Fn1, fibronectin 1; Sham, sham-operated group; BC, background control group; Nx, 5/6 nephrectomy; UCG, uremic clearance granule; HGF-L, Huang Gan formula low dose group; HGF-M, Huang Gan formula middle dose group; HGF-H, Huang Gan formula high dose group.