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. Author manuscript; available in PMC: 2015 Jun 9.
Published in final edited form as: Neurobiol Dis. 2008 Nov 6;33(3):315–330. doi: 10.1016/j.nbd.2008.10.009

Fig. 5.

Fig. 5

Merged CLSM images showing the location of aggregates in cortex and striatum of a 12 week old R6/2 mouse and a 23 week old R6/2x mouse, with respect to cell nuclei. Aggregates were detected by immunolabeling for the N-terminus of huntingtin using the EM48 antibody (as visualized with ALEXA594-conjugated donkey anti-mouse IgG), while neuronal nuclei were detected with the Sytox nuclear stain. Immunolabeling detects aggregated (red) mutant protein in Sytox-stained (green) neuronal nuclei in R6/2 mice but in the neuropil in R6/2x mice. These results indicate that the expanded mutant transgene protein avoids nuclear entry and predominantly forms extranuclear aggregates in R6/2x mice. Both mice were sacrificed at morbidity. Magnification is the same in all images.