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. Author manuscript; available in PMC: 2015 Jun 9.
Published in final edited form as: Eur J Neurosci. 2008 Dec;28(11):2213–2220. doi: 10.1111/j.1460-9568.2008.06514.x

FIG. 4.

FIG. 4

Calcium responses obtained in Bergmann glial cells from acute cerebellar slices. (A) Simple peak-type calcium responses in Fura-2-loaded Bergmann glial cells to local pipette application of CGRP (2.5 μM) and ATP (100 μM). Note that the onset of decay of the CGRP response occurreds in the presence of the peptide. The slice was prepared from a P4 mouse. (B) Peak plus plateau-type calcium responses in Bergmann glial cells to local pipette application of 50 μM CGRP. The slice was prepared from a P6 mouse cerebellum. Note that recovery from the plateau starts with cessation of CGRP application. (C, D) Calcium responses recorded from cell body and process of a single Bergmann glia cell from a cerebellar slice of a P3 mice. In (C) the fluorescence photograph of a Fura-2 loaded Bergmann glia cells is shown. The cell body and radial process (Bergmann glia fiber) of the analyzed cell has been separately outlined: 0 = cell body; 1 = radial process. In (D) the ratiometric traces of calcium concentration in the corresponding cell domains following local pipette application of CGRP (50 μM; indicated by bar) is shown.