Figure 1.

Expression, crystallization and actin-severing activity of recombinant human cofilin 1. (a) Polyacrylamide gel electrophoresis of wild-type (WT) and four cysteine-to-alanine point-mutant forms of purified recombinant human cofilin 1. (b) C147A cofilin 1 crystals. (c) Fluorescence of pyrene-labelled filamentous actin without the addition of cofilin 1 (F-actin) or with the addition of WT or C147A cofilin 1 as indicated over time. The decrease in fluorescence indicates a shift in the proportion of F-actin towards G-actin. Lines represent the average of 8–12 determinations. (d) Means and standard deviation of the fluorescence remaining at the 300 s experiment endpoint for the indicated conditions for 8–12 determinations.