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. 2004 Aug;78(15):8238–8244. doi: 10.1128/JVI.78.15.8238-8244.2004

FIG. 3.

FIG. 3.

MuAPOBEC3Gwt worked synergistically with huAPOBEC3G on the infectivity of HIV-1 virions but not on MLV virions. (A) We transfected pNL43-Luc (wild type) or pNL43/Δvif-Luc (Δvif) with a combination of expression vectors for APOBEC3G (increasing amounts of muAPOBEC3Gwt or huAPOBEC3G with a fixed amount of huAPOBEC3G or muAPOBEC3Gwt, respectively) into HEK293T cells. An infectivity assay was performed, and values are presented as described in the legend to Fig. 1. (B) We transfected pDON/Luc with a combination of expression vectors for APOBEC3G as described in A into GP293 cells. An infectivity assay was carried out, and values are presented as described in the legend to Fig. 2A. (C) A coimmunoprecipitation assay revealed the physical interaction between huAPOBEC3G and muAPOBEC3Gwt. We transfected pcDNA3/HA-based vectors with pDON/EGFP-based vectors. Cell lysates were immunoprecipitated (IP) with anti-EGFP monoclonal antibody (Ab) and subjected to immunoblotting with anti-HA monoclonal antibody. Solid arrow, huAPOBEC3G; open arrow, muAPOBEC3Gwt.