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. 2004 Aug;78(15):8238–8244. doi: 10.1128/JVI.78.15.8238-8244.2004

FIG. 5.

FIG. 5.

MuΔexon5 and muAPOBEC3Gwt showed similar antiviral effects. (A) We transfected pNL43-Luc (wild type) or pNL43/Δvif-Luc (Δvif) with pcDNA/HA-based vectors into HEK293T cells with the indicated amount of plasmid. An infectivity assay was carried out, and values are presented as described in the legend to Fig. 1. MuΔexon5 and muAPOBEC3Gwt showed similar antiviral effects. (B) Protein expression of APOBEC3G in producer cells (upper column) and incorporation of APOBEC3G into virions (lower column) were detected by Western blot analysis with anti-HA monoclonal antibody. The expression of β-actin and the incorporation of p24 were used as internal controls for protein expression in the cell lysate and protein incorporation into virions, respectively. The expression of HIV-1 Vif protein reduced the protein expression of huAPOBEC3G in producer cells as well as the incorporation of huAPOBEC3G into HIV-1 virions, while it did not affect the protein expression or incorporation of muAPOBEC3Gwt and muΔexon5 into virions.