FIG. 6.

MuAPOBEC3G (muAPOBEC3Gwt or muΔexon5) did not show antiviral effects on MLV because it was not incorporated into MLV virions. We transfected pDON-Luc and pVSV-G with the indicated amount of pcDNA3/HA-based vectors into GP293 cells. An infectivity assay was performed, and values are presented as described in the legend to Fig. 2A. (A) HuAPOBEC3G suppressed the infectivity of MLV, but muAPOBEC3G did not. (B) Protein expression of APOBEC3G in producer cells (upper column) and the incorporation of APOBEC3G into virions (lower column) were detected by Western blot analysis with anti-HA monoclonal antibody. MuAPOBEC3G was not incorporated into MLV virions, although it was expressed in producer cells at a level comparable to huAPOBEC3G. Solid arrow, huAPOBEC3G or muΔexon5; open arrow, muAPOBEC3Gwt. (C) The expression of muAPOBEC3G was not impaired by the expression of MLV.