Table 3. 3SP-CoA desulfination activity of AcdDPN7 mutants.
ND, not determined.
| AcdDPN7 | K m (mM) | Specific activity (molmin1mg1) | k cat (s1) | k cat/K m (s1mM 1) |
|---|---|---|---|---|
| Wild type (Schrmann et al., 2013 ▶) | 0.013 0.001† | 4.19 0.07† | 3.13 0.05† | 237.2 4.1† |
| R84K | ND | 0.00 0.00‡ | 0.00 0.00‡ | ND |
| Q246E | 0.019 0.000 | 5.26 0.05 | 3.93 0.04 | 203.2 1.9 |
†
Standard deviations which were not given in the original publication were recalculated based on the raw data. The k cat/K m value reported here differs from the original value owing to rounding errors in the original publication which are now omitted.
‡
No enzyme activity was observed even when the purified enzyme was applied at 65gml1, while 2gml1 enzyme was applied in the assays with the wild-type enzyme and the AcdQ246E mutant. Measurements were performed in triplicate.