Extended Data Figure 5. CK2 regulates transcriptional elongation.

(a) CK2 kinase activity is necessary for normal gene expression. LNCaP cells were treated with vehicle (DMSO) or TBBz (25 µM) for 60 minutes, and then treated with vehicle (EtOH) or 100 nM DHT for 90 min, and induction of the indicated AR target genes was measured by RT-qPCR (n=3, mean ± SEM, * indicates p<0.05, ** indicates p<0.01). P-values were calculated by Student’s two-tailed t-test. (b) Nuclear extracts from 293T cells were immunoprecipitated using CK2α antibody and immunoblotted. (c) Enrichment of CK2α, H3K4me1¹⁸, H3K27acetyl¹⁸ and AR¹⁸ at a representative AR enhancer (KLK3) is shown. (d) Pol II tag density in cells treated with vehicle or TBBz at a representative RHOU enhancer is shown. Data represent two (b–d) or three independent experiments (a).