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. Author manuscript; available in PMC: 2015 Jun 9.
Published in final edited form as: DNA Repair (Amst). 2013 Dec 4;13:10–21. doi: 10.1016/j.dnarep.2013.10.011

Fig. 1.

Fig. 1

HeLa cells with hMYH-knockdown are more sensitive to H2O2 than control. (A) mRNA level of hMYH is reduced to 12% in knockdown (KD) cells as compared to non-target (NT) control cells. HeLa cells were stably transfected with lentivirus containing shRNA against hMYH or control shRNA. mRNA levels were determined by RT-qPCR. (B) The protein level of hMYH is reduced to 30% in knockdown cells as compared to control cells. Extracts were prepared from control and knockdown cells and Western blotting were analyzed with hMYH and β-actin antibodies. (C) Sensitivity to H2O2 of MYH KD cells. Both non-target and MYH knockdown HeLa cells were treated with H2O2 at indicated concentrations for 1 hr. The survived cells were detected after 24 hrs. Survival differences are statistical significant between two cell lines at 100-500 μM H2O2.