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. Author manuscript; available in PMC: 2015 Jun 9.
Published in final edited form as: DNA Repair (Amst). 2013 Dec 4;13:10–21. doi: 10.1016/j.dnarep.2013.10.011

Fig. 3.

Fig. 3

HeLa cells with hMYH knockdown are more sensitive to apoptosis and contain higher level of 8-oxoG than control cells after H2O2 treatment. HeLa cells were stably transfected with lentivirus containing shRNA against hMYH or non-target (NT) shRNA. (A) Cells were treated with 150 μM H2O2 for 1 hr, replaced with fresh media for one day, and subjected to DAPI staining and TUNEL assay. Both were merged in the third column. (B) Cells were treated with 150 μM H2O2 for 1 hr and subjected to immuno-florescent staining with anti-8-oxo-dG antibody. Nuclear DNA was counterstained with DAPI. Upper panel, cells with control NT shRNA. Lower panel was from cells with hMYH knockdown. (C) Quantitative analyses of GO accumulation of NT (white bar) and MYH-ND (black bars) cells from three experiments. The percentage (%) of cells containing GO is presented after H2O2 treatment. The error bars reported are the standard deviations of the averages and P-value shown was calculated by Student's t-test.