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Effect of N-linked glycan removal on PRRSV infection of PAM. PRRSV (▪) or PAM (□) were treated with N-glycosidase F (A) or endoglycosidase H (B) before infection of PAM. After treatment, PAM were inoculated for 1 h at 37°C with PRRSV, washed to remove unbound virus, and cells were fixed 10 h postinoculation Data represent means ± standard deviation (error bars) of triplicate assays.
