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Flow cytometric analysis of PRRSV attachment to PAM. Biotinylated PRRSV was incubated for 90 min at 37°C with different concentrations of V. cholerae neuraminidase. PAM were then incubated for 1 h at 4°C with enzyme-treated virus. After washing, bound virus particles were stained with fluorescein isothiocyanate-labeled streptavidin, and fluorescence intensity was analyzed by flow cytometry. Data represent means ± standard deviation (error bars) of triplicate assays.
