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HIV-1 Nef protein induces cyclin T1 in MDMs. MDMs from donors I and J were infected at day 23 of differentiation with Ad5-GFP or Ad5-Nef at multiplicities of infection of 1,000 (donor I) or 100 (donor K), and cell extracts were prepared at day 25. Donor K was infected at day 16 of differentiation at a multiplicity of infection of 50, and cell extracts were prepared at day 18. Cell extracts were analyzed by immunoblotting.
