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Gα12/13 enhances US28-mediated SMC migration. PASMCs were infected with HCMV and adenoviruses (Ad) expressing Gαq, Gα11, Gα12, Gα13, or Gαi1. Expression of the Gα proteins was normalized by Western blotting, and the MOI was adjusted accordingly such that equivalent amounts of protein were expressed. The total number of cells migrating from the upper to lower chamber was enumerated at 48 to 72 h postinfection.
