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Reconstitution of ΔM50 constructs
| BAC | Genea | Plaque formationb |
|---|---|---|
| pSM3fr | M50 | +++ |
| pΔm152/GFP | M50 | +++ |
| pSMfr-ΔM50 | ΔM50 | − |
| pΔM50-GFP | ΔM50 | − |
| pΔM50-GFP + pCR3-M50Flag | ΔM50 + M50 in trans | (+) |
| pM50E | ΔM50 + M50 ectopic | +++ |
M50 expression and M50/p35 in trans.
Formation of viral plaques 10 days postinfection. +++, wt plaque formation; −, no plaque formation; (+), formation of miniplaques.
