FIG. 2.

The anti-pE120R antibody recognizes newly assembled virions. Vero cells were infected with the Ba71v strain of ASFV, fixed at the indicated time, and processed for immunofluorescence. Samples were incubated with a rabbit antiserum raised against the late ASFV structural protein pE120R (top panels) and a mouse antibody against the early viral protein p30 (middle panels). Viral DNA and cellular DNA were labeled with DAPI (bottom panels). The ASFV structural protein pE120R is not detected until 12 hpi or not at all in the presence of Ara-C, an inhibitor of late gene expression. Scale bar, 20 μm.