Fig 4. Islet endocrine cell and β-cell area are profoundly reduced in pancreata of diabetic dogs.

(a-b) Quantification of (a) islet endocrine (synaptophysin⁺) cross-sectional area and (b) β-cell (insulin⁺) cross-sectional area, expressed as % total pancreas area per section. (c-d) Comparison of insulin⁺ and synaptophysin⁺ area in (c) control dogs and (d) diabetic dogs. (a-b) mean ± SEM for 14 control and 21 diabetic dogs. ***, p<0.001; ****, p<0.0001. Synaptophysin area and insulin area are correlated in (c) controls (R² = 0.9680, p<0.0001) and (d) diabetics (R² = 0.3679, p = 0.017) (e-f) Islet endocrine cell area and β-cell area are not correlated with duration of diabetes. Duration of diabetes vs. islet endocrine area (e) or β-cell area (f). Blood glucose vs. islet endocrine cell area (g) or β-cell area (h). Islet endocrine cell area or β-cell area expressed as % total pancreas area per section. Blood glucose is correlated with islet endocrine cell area (R² = 0.26, P<0.05) and β-cell area (R² = 0.31, P<0.05).