Fig 5. The knockdown of MATP acidifies the melanosomal pH.

(A) MNT-1 cells stably expressing scrambled or MATP shRNAs were treated with the pH indicator DAMP for 30 min. An anti-DNP antibody was used to detect DAMP by fluorescence microscopy. Scale bars = 200 μm. (B) The fluorescence intensity was calculated with ImageJ software (http://rsbweb.nih.gov/ij/download.html). The data are representative of three independent experiments (*, P < 0.05). (C) MNT-1 cells stably expressing scrambled or MATP shRNAs were co-stained with anti-DNP and anti-HMB45 antibodies after DAMP treatment for 30 min and then visualized by confocal microscopy. Each inset was magnified. Scale bars = 10 μm.