FIG. 5.

Wee-1 depletion by siRNA Wee-1 alleviates Vpr-induced arrest. HeLa cells (1.5 × 10⁵) were synchronized at G₁/S by double thymidine block and transfected with siWee-1 or small interfering luciferase (siLuc.). Cells were then infected with HR′Vpr or HR′Thy virus (0.3 μg of viral p24). (A) Cell cycle profiles were determined at various time points following infection. P.R., postrelease. (B) Fourteen hours after infection whole-cell lysates were prepared and analyzed by Western blotting.