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. 2015 Jun 9;10(6):e0128185. doi: 10.1371/journal.pone.0128185

Fig 4. Binding of FHbp to immobilized human FH by surface plasmon resonance.

Fig 4

Purified human FH (1500 response units) was coupled to the biosensor chip. Different concentrations (3 to 316 nM) of purified, recombinant FHbp were used to determine equilibrium dissociation constants (Table 1). A. Data for injection of 100 nM of wild-type (WT) FHbp (grey line) or Q38R mutant (black line) are shown. B-E. Data for E92K, R130G, S223R and H248L mutants are shown with WT FHbp (same data as in Panel A). Binding equivalent to two response units was considered as the limit of detection.