Table 1. Macromolecule-production information.
Source organism | S. agalactiae |
DNA source | Genomic DNA |
Forward primer | TTAAATATTTATCTATTACCTCCTAGTAGCGAACGTTATGG |
Reverse primer | ATGGCTGCCGCGCGGCACCAG |
Cloning vector | pET-28b |
Expression vector | pET-28b |
Expression host | E. coli BL21 (DE3) |
Complete amino-acid sequence of the construct produced | MGSSHHHHHHHHSSGLVPRGSHLNIYLLPPSSERYGRVILDRVEQRGLYSQGRQWQIIRQRSEKKLKTSKSYQESRNIVQEAVRYGGGKHSQILSKETVRRDTLDSRYPEYRRLNEDILLITIPSISKLDKRSISHYSGKLQNILMEKSYKGLILDLSNNTGGNMIPMIGGLASILPNDTLFHYTDKYGNKKTITMKNIPLEALKISRKTINTKHVPIAIITNHKTASSAEMTFLSFKGLPNVKSFGQATAGYTTVNETFMLYDGARLALTTGIVSDRQGYKYENTPILPDQVTSLPLQESQSWLKSRINQN |