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. 2015 May 20;71(Pt 6):671–675. doi: 10.1107/S2053230X15006226

Table 1. Macromolecule-production information.

Source organism S. agalactiae
DNA source Genomic DNA
Forward primer TTAAATATTTATCTATTACCTCCTAGTAGCGAACGTTATGG
Reverse primer ATGGCTGCCGCGCGGCACCAG
Cloning vector pET-28b
Expression vector pET-28b
Expression host E. coli BL21 (DE3)
Complete amino-acid sequence of the construct produced MGSSHHHHHHHHSSGLVPRGSHLNIYLLPPSSERYGRVILDRVEQRGLYSQGRQWQIIRQRSEKKLKTSKSYQESRNIVQEAVRYGGGKHSQILSKETVRRDTLDSRYPEYRRLNEDILLITIPSISKLDKRSISHYSGKLQNILMEKSYKGLILDLSNNTGGNMIPMIGGLASILPNDTLFHYTDKYGNKKTITMKNIPLEALKISRKTINTKHVPIAIITNHKTASSAEMTFLSFKGLPNVKSFGQATAGYTTVNETFMLYDGARLALTTGIVSDRQGYKYENTPILPDQVTSLPLQESQSWLKSRINQN