Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
| Method | Sitting-drop vapour diffusion |
| Plate type | 96-well plate |
| Temperature (K) | 294 |
| Protein concentration (M) | 300 |
| Buffer composition of protein solution | 20mM TrisHCl pH 7.5, 150mM NaCl, 1mM BME |
| Composition of reservoir solution | 20% PEG 3000, 0.1M HEPES pH 7.5, 0.2M NaCl |
| Volume and ratio of drop | 0.4l (1:1) |
| Volume of reservoir (l) | 0.4 |
