FIG. 4.

CAT expression in minigenome replication assays in 293T cells. 293T cells were transfected with a T7 RNA polymerase-expressing plasmid, plasmids pCITE-L, pCITE-N, pCITE-P, and pCITE-M2.1, and the minigenome vector of either NL/1/00 (left panel) or NL/1/99 (right panel). Each of the individual plasmids was omitted from the transfection mixture to test the requirement of each individual component. Cell lysates were harvested 48 h after transfection, and levels of CAT were determined by enzyme-linked immunosorbent assay.