TABLE 1.
Determination of leader sequences of hMPV NL/1/99 and NL/1/00 obtained by RT-PCR following ligation of viral RNA and 3′ RACEa
| Leader sequence positive strand | NL/1/99 RT-PCR circles (n = 26) | NL/1/00 RT-PCR circles (n = 35) | NL/1/00 poly(A) tailing and 3′ RACE (n = 79) |
|---|---|---|---|
| ACGCGAAAAAAACGCG | 0 (0) | 0 (0) | 15 (19) |
| CGCGAAAAAAACGCG | 0 (0) | 0 (0) | 6 (8) |
| GCGAAAAAAACGCG | 15 (58) | 30 (85) | 5 (6) |
| CGAAAAAAACGCG | 6 (23) | 2 (6) | 23 (29) |
| GAAAAAAACGCG | 2 (8) | 1 (3) | 0 (0) |
| AAAAAAACGCG | 2 (8) | 0 (0) | 0 (0) |
| Larger deletion | 1 (4) | 2 (6) | 30 (38) |
a
All data given as number (%).