Table 1. Macromolecule-production information.
Source organism | M. tuberculosis H37Rv |
DNA source | M. tuberculosis H37Rv genome |
Forward primer | GGAATTCCATATGGTGCTTGACAGCGATGCGCG |
Reverse primer | CCCAAGCTTTCACGGGCTTTCGTCGACCAG |
Cloning vector | pET-28a |
Expression vector | pET-28a |
Expression host | E. coli BL21(DE3) |
Complete amino-acid sequence of the construct produced | MGSSHHHHHHSSGLVPRGSHMVLDSDARLASDLSLAVMRLSRQLRFRNPSSPVSLSQLSALTTLANEGAMTPGALAIRERVRPPSMTRVIASLADMGFVDRAPHPIDGRQVLVSVSESGAELVKAARRARQEWLAERLATLNRSERDILRSAADLMLALVDESP |