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. 2015 May 22;71(Pt 6):741–745. doi: 10.1107/S2053230X15007281

Table 1. Macromolecule-production information.

Source organism M. tuberculosis H37Rv
DNA source M. tuberculosis H37Rv genome
Forward primer GGAATTCCATATGGTGCTTGACAGCGATGCGCG
Reverse primer CCCAAGCTTTCACGGGCTTTCGTCGACCAG
Cloning vector pET-28a
Expression vector pET-28a
Expression host E. coli BL21(DE3)
Complete amino-acid sequence of the construct produced MGSSHHHHHHSSGLVPRGSHMVLDSDARLASDLSLAVMRLSRQLRFRNPSSPVSLSQLSALTTLANEGAMTPGALAIRERVRPPSMTRVIASLADMGFVDRAPHPIDGRQVLVSVSESGAELVKAARRARQEWLAERLATLNRSERDILRSAADLMLALVDESP