Table 1. Crystallization of active and latent cgAUS1.
| Crystallization condition (resulting data set) | A (1, 2) | B (3) | C (4) | D (5) | E (6) | F † (7) |
|---|---|---|---|---|---|---|
| Protein sample | cgAUS1-a1, cgAUS1-a2 | cgAUS1-a2 | cgAUS1-ln | cgAUS1-lr | cgAUS1-lr | cgAUS1-lr |
| Protein concentration (mgml1) | 36 | 6 | 10 | 14 | 14 | 14 |
| Composition of reservoir solution | 2325% PEG 4000, 500mM NaCl, 100mM Na formate, 50mM Na citrate pH 6.4 | 22% PEG 4000, 500mM NaCl, 100mM Na formate, 50mM Na citrate pH 6.4, 100mM 1,4-resorcinol | 15% PEG 4000, 100mM MgCl2, 60mM Na citrate pH 7.4 | 15% PEG 4000, 100mM MgCl2, 60mM Na citrate pH 7.4 | 12% PEG 4000, 60mM Na citrate pH 6.4, 1mM Na6[TeW6O24] | 15% PEG 4000, 60mM Na citrate pH 5.0, 20% glycerol, 1mM Na6[TeW6O24] |
| Volume of drop (l) | 2, 3 | 2 | 2 | 2 | 2 | 2 |
| Ratio of drop (protein:reservoir) | 1:1; 1:2 | 1:1 | 1:1 | 1:1 | 1:1 | 1:1 |
| Cryoprotectant solution | 40% PEG 4000, 15% glycerol, 20mM Na citrate pH 6.4 | 40% PEG 4000, 15% glycerol, 20mM Na citrate pH 6.4, 100mM 1,4-resorcinol | 40% PEG 4000, 15% glycerol, 20mM Na citrate pH 7.4 | 40% PEG 4000, 15% glycerol, 20mM Na citrate pH 7.4 | 40% PEG 4000, 15% glycerol, 20mM Na citrate pH 6.4 | 40% PEG 4000, 15% glycerol, 20mM Na citrate pH 5.0, 5mM H2O2 |
†
The cryoprotectant solution contained hydrogen peroxide to generate the oxy-form of the dinuclear copper site.