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| Method | Microbatch-under-oil; hanging-drop vapour diffusion |
| Plate type | 72-well flat bottom type; 24-well plates |
| Temperature (K) | 293 |
| Protein concentration (mgml1) | 10 |
| Buffer composition of protein solution | 20mM TrisHCl pH 8.5, 200mM NaCl, 5% glycerol |
| Composition of reservoir solution | 200mM CaCl2, 100mM TrisHCl pH 7.5, 22% PEG 4000 |
| Volume and ratio of drop | 2l (1:1); 4l (1:1) |
| Volume of reservoir (l) | 0 (microbatch); 400 (vapour diffusion) |
