Table 1. Macromolecule-production information.
Sequence of wild-type CsuA/B† | MNMKNIQKSLLAALIVAGYAVNTQAAVTGQVDVKLNISTGCTVGGSQTEGNMNKFGTLNFGKTSGTWNNVLTAEVASAATGGNISVTCDGTDPVDFTVAIDGGERTDRTLKNTASADVVAYNVYRDAARTNLYVVNQPQQFTTVSGQATAVPIFGAIAPNTGTPKAQGDYKDTLLVTVNF |
Modified sequence of CsuA/B‡ | MNMKNIQKSLLAALIVAGYAVNTQAAVTHHHHHHSTGCTVGGSQTEGNMNKFGTLNFGKTSGTWNNVLTAEVASAATGGNISVTCDGTDPVDFTVAIDGGERTDRTLKNTASADVVAYNVYRDAARTNLYVVNQPQQFTTVSGQATAVPIFGAIAPNTGTPKAQGDYKDTLLVTVNF |
Sequence of CsuC§ | MVICMNNSAFIKNGILKSFLFASTLSLVTPVMAQATFLIWPIYPKIEANEKATAVWLQNTGKTDAMVQIRVFKWNQDGLKDNYSEQSEIIPSPPVAKIKAGEKHMLRLTKSVNLPDGKEQSYRLIVDELPIRLSDGNEQDASKVSFQMRYSIPLFAYGKGIGSGLTEESQKLNAKNALAKPVLQWSVRNNQQGQSELYLKNNGQKFARLSALKTSKTGNDISLGKAAFGYVLSNSTVKFAIDQSTAHELAKTSKIYGVDSSGIKQELIEITKMEDPS |
Source organism | A. baumannii |
DNA source | Oligonucleotide synthesis |
Cloning vector | pUC57 |
Expression vector | pET101-CsuC-6HCsuA/B |
Expression host | E. coli strains BL21-AI and BL21(DE3) (Invitrogen) |
The secretion signal sequence is shown in italics and hypothetical hydrophobic donor-strand residues are underlined.
The 6His tag is underlined.
The secretion signal sequence is shown in italics.