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. 2015 May 22;71(Pt 6):770–774. doi: 10.1107/S2053230X15007955

Table 1. Macromolecule-production information.

Sequence of wild-type CsuA/B MNMKNIQKSLLAALIVAGYAVNTQAAVTGQVDVKLNISTGCTVGGSQTEGNMNKFGTLNFGKTSGTWNNVLTAEVASAATGGNISVTCDGTDPVDFTVAIDGGERTDRTLKNTASADVVAYNVYRDAARTNLYVVNQPQQFTTVSGQATAVPIFGAIAPNTGTPKAQGDYKDTLLVTVNF
Modified sequence of CsuA/B MNMKNIQKSLLAALIVAGYAVNTQAAVTHHHHHHSTGCTVGGSQTEGNMNKFGTLNFGKTSGTWNNVLTAEVASAATGGNISVTCDGTDPVDFTVAIDGGERTDRTLKNTASADVVAYNVYRDAARTNLYVVNQPQQFTTVSGQATAVPIFGAIAPNTGTPKAQGDYKDTLLVTVNF
Sequence of CsuC§ MVICMNNSAFIKNGILKSFLFASTLSLVTPVMAQATFLIWPIYPKIEANEKATAVWLQNTGKTDAMVQIRVFKWNQDGLKDNYSEQSEIIPSPPVAKIKAGEKHMLRLTKSVNLPDGKEQSYRLIVDELPIRLSDGNEQDASKVSFQMRYSIPLFAYGKGIGSGLTEESQKLNAKNALAKPVLQWSVRNNQQGQSELYLKNNGQKFARLSALKTSKTGNDISLGKAAFGYVLSNSTVKFAIDQSTAHELAKTSKIYGVDSSGIKQELIEITKMEDPS
Source organism A. baumannii
DNA source Oligonucleotide synthesis
Cloning vector pUC57
Expression vector pET101-CsuC-6HCsuA/B
Expression host E. coli strains BL21-AI and BL21(DE3) (Invitrogen)

The secretion signal sequence is shown in italics and hypothetical hydrophobic donor-strand residues are underlined.

The 6His tag is underlined.

§

The secretion signal sequence is shown in italics.