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| Method | Hanging-drop vapour diffusion |
| Plate type | 24-well hanging-drop plate (Hampton Research) |
| Temperature (K) | 289 |
| Protein concentration (mgml1) | 23 |
| Buffer composition of protein solution | 25mM HEPES, 150mM NaCl pH 7.2 |
| Composition of reservoir solution | 0.1M sodium malonate pH 7.0, 15% PEG 3350 |
| Volume and ratio of drop | 4l, 1:1 |
| Volume of reservoir (l) | 1000 |
