| Isoform | 1.9 | 6.9 | 6.44 | 4.44 | 7.44 |
|---|---|---|---|---|---|
| Method | Hanging drop | Hanging drop | Hanging drop | Hanging drop | Hanging drop |
| Plate type | 24-well | 24-well | 24-well | 24-well | 24-well |
| Temperature (K) | 289 | 289 | 289 | 289 | 289 |
| Protein concentration (mgml1) | 15 | 15 | 13 | 12 | 10 |
| Composition of reservoir solution | 0.1M bis-tris pH 5.5, 0.1M ammonium acetate, 20%(w/v) PEG 10000 | 0.1M HEPES pH 7.5, 0.2M ammonium sulfate, 25%(w/v) PEG 3350 | 0.1M HEPES pH 7.5, 15%(w/v) PEG 3350 | 0.1M HEPES pH 7.5, 0.1M calcium chloride, 30%(w/v) PEG 400 | 0.2M ammonium chloride, 17%(w/v) PEG 3350 |
| Volume and ratio of drop | 1:1 | 1:1 | 1:1 | 1:1 | 1:1 |
| Volume of reservoir (l) | 500 | 500 | 500 | 500 | 500 |