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. 2015 May 22;71(Pt 6):775–778. doi: 10.1107/S2053230X15008201
Isoform 1.9 6.9 6.44 4.44 7.44
Method Hanging drop Hanging drop Hanging drop Hanging drop Hanging drop
Plate type 24-well 24-well 24-well 24-well 24-well
Temperature (K) 289 289 289 289 289
Protein concentration (mgml1) 15 15 13 12 10
Composition of reservoir solution 0.1M bis-tris pH 5.5, 0.1M ammonium acetate, 20%(w/v) PEG 10000 0.1M HEPES pH 7.5, 0.2M ammonium sulfate, 25%(w/v) PEG 3350 0.1M HEPES pH 7.5, 15%(w/v) PEG 3350 0.1M HEPES pH 7.5, 0.1M calcium chloride, 30%(w/v) PEG 400 0.2M ammonium chloride, 17%(w/v) PEG 3350
Volume and ratio of drop 1:1 1:1 1:1 1:1 1:1
Volume of reservoir (l) 500 500 500 500 500