| Isoform | 9.44, Zn | 9.44, no Zn | 4.4 | 8.4 |
|---|---|---|---|---|
| Method | Hanging drop | Hanging drop | Hanging drop | Hanging drop |
| Plate type | 24-well | 24-well | 24-well | 24-well |
| Temperature (K) | 289 | 289 | 289 | 289 |
| Protein concentration (mgml1) | 10 | 10 | 11 | 15 |
| Composition of reservoir solution | 0.1M sodium cacodylate pH 6.4, 0.2M zinc acetate, 20%(w/v) PEG 4000 | 0.1M sodium acetate pH 4.6, 25%(w/v) PEG 1500 | 0.1M HEPES pH 7.5, 0.5M magnesium chloride, 20%(w/v) PEG 3350 | 0.1M MES pH 6.5, 20%(w/v) PEG 3350 |
| Volume and ratio of drop | 1:1 | 1:1 | 1:1 | 1:1 |
| Volume of reservoir (l) | 500 | 500 | 500 | 500 |
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