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. 2015 Jun 9;10(6):e0130165. doi: 10.1371/journal.pone.0130165

Fig 2. Western blotting analysis of transferrin-AlexaFluor 633 uptake in isolated Trypanosoma cruzi intracellular amastigotes.

Fig 2

(A) Detection of holo-transferrin (using anti-transferrin goat antiserum) in total protein extracts of isolated intracellular amastigotes and epimastigotes (positive control for endocytosis) endocytosis assays at different temperatures. The panel on the left shows the Ponceau-stained membrane used for transferrin immuno-blotting analysis (on the right). The protein band with approximately 80 kDa corresponds to transferrin. Protein extract from isolated intracellular amastigotes incubated with transferrin at 37°C showed a strong band, indicating transferrin internalization. TcGAPDH (~40 kDa) was used as a loading control. (B) Ratios between the integrated densities of the transferrin band at 28 or 37°C and 4°C (Y-axis), based on measurements performed using ImageJ.