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. 2015 Jun 9;65(6):562–570. doi: 10.1097/FJC.0000000000000224

FIGURE 4.

FIGURE 4

Analysis of NO production (A) and eNOS activity (C) and arterial signaling pathways in Ctr or MCT-treated rats without or with short-term CR. Protein expression is shown in pulmonary artery tissues for eNOS (B), eNOS phosphorylated at serine 1177 (p-eNOS) (D), acetylated eNOS (E), and SIRT-1 (F). Protein expression of eNOS, p-eNOS, and SIRT-1 is expressed relative to β-actin and immunoprecipitated acetylated eNOS is expressed relative to control eNOS expression. Representative images are shown above the summary data. Data are shown normalized to the control mean values. Values are mean values ± standard error of the mean (n = 6). *P < 0.05, **P < 0.01 versus Ctr and #P < 0.05, ##P < 0.01 versus MCT using a 1-way ANOVA with Bonferroni multiple comparisons test.