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. 2015 Jun 9;65(6):562–570. doi: 10.1097/FJC.0000000000000224

FIGURE 5.

FIGURE 5

Effect of SIRT1 overexpression on MCT-induced pulmonary hypertension and endothelial dysfunction. Intratracheal administration of Ad.SIRT1 resulted in increased SIRT-1 expression (A) and phosphorylated eNOS (B) and decreased acetylated eNOS (C) in pulmonary artery tissues form MCT-treated rats at 8 days after transfection compared with Ad.GFP administration (1 week after MCT injection). SIRT-1 is expressed relative to β-actin. Phosphorylated eNOS and immunopreciptated acetylated eNOS are expressed relative to total or control eNOS expression. Data are shown normalized to the control mean values. Overexpression of SIRT1 decreased mPAP (D) and improved concentration-dependent vasorelaxation responses to Ach (E) at 3 weeks after MCT injection. No significant difference existed in concentration-dependent vasorelaxation responses to NaNO2 (F) between Ad.SIRT1 and Ad.GFP administration at 3 weeks after MCT injection. Values are mean values ± standard error of the mean (n = 6). *P < 0.05, **P < 0.01 versus MCT + Ad.GFP 1w or 3w using an unpaired Student 2-tailed t test.