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. 2015 Jun 9;65(6):579–586. doi: 10.1097/FJC.0000000000000226

FIGURE 3.

FIGURE 3

TXL increases the expression of tight junction proteins and KLF4 in hypoxic aorta. A, Endothelial cells were treated with the indicated stimuli for 24 hours. Western blot analysis of VE-cadherin, beta-catenin, occludin, claudin-1, ZO-1, and KLF4 expression is shown (left panel). Densitometric scanning (right panel). Values are the mean ± SD from 3 independent experiments. *P < 0.05, compared with the Con. B, Endothelial cells were treated with the indicated stimuli for 24 hours. Total RNA was prepared, and the mRNA levels of VE-cadherin, beta-catenin, occludin, claudin-1, and KLF4 were examined by quantified RT-PCR and normalized to GAPDH. The data are presented as mean ± SD. *P < 0.05, #P < 0.05. C, The thoracic aortas of the 3 groups were isolated. TJ protein and KLF4 expression levels were evaluated by immunohistochemical staining using antioccludin, anti–claudin-1, and anti-KLF4 antibodies (×100). Arrows indicate localization of TJ protein in the enlarged panels (×200). D, The thoracic aortas of the 3 groups were isolated. TJ protein and KLF4 expression levels were evaluated by Western blotting using antioccludin, anti–claudin-1, and anti-KLF4 antibodies. E, Endothelial cells were treated with the indicated stimuli for 24 hours. Distribution of occludin, claudin-1, and ZO-1 expression was detected using immunofluorescence staining.