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. 2015 Jun 9;65(6):579–586. doi: 10.1097/FJC.0000000000000226

FIGURE 4.

FIGURE 4

KLF4 may mediate TXL-induced expression of the tight junction proteins occludin and claudin-1. A, Endothelial cells were subjected to various stimuli for 24 hours. Western blot analysis was used to detect the expression of KLF4, VE-cadherin, beta-catenin, occludin, ZO-1, and claudin-1. Densitometric scanning (right panel). Values are the mean ± SD from 3 independent experiments. *P < 0.05, compared with the pAd group. B, Endothelial cells were subjected to various stimuli for 24 hours. Total RNA was prepared, and the mRNA levels of KLF4, VE-cadherin, beta-catenin, occludin, and claudin-1 were examined by qRT-PCR and normalized to GAPDH. The data are presented as mean ± SD from 3 independent experiments. *P < 0.05, #P < 0.05. C, Endothelial cells were treated with the indicated stimuli for 24 hours. Distribution of claudin-1 and ZO-1 expression was detected using immunofluorescence staining. D, TXL-induced KLF4 phosphorylation was detected by immunoprecipitation using an anti-phosphoserine antibody and immunoblotting with the anti-KLF4 antibody.