Figure 3.
Comparison of the effects of hypoxia (2% O2) and 4AP (10 mm) on PAP in control and perinatally hyperoxic rats
A, representative recording obtained in a control rat. B, representative recording obtained in a perinatally hyperoxic rat. C, mean ± SD of the increase in PAP elicited by hypoxia and 4AP in control (n = 12) and perinatally hyperoxic rats (n = 8). **P < 0.01. D, representative immunostaining of a section of the small arteries belonging to control and perinatally hyperoxic rats and a blank obtained by elimination of the primary antibody. D, procedure used to calculate the thickness of the muscular layer as the mean of five (or more) individual thickness measured on the images. E, mean thickness in eight control and eight hyperoxic small arteries of 200–300 μm [17.3 ± 1.9 (95% CI = 15.72–18.93) vs. 16.9 ± 1.2 (95% CI = 15.86–17.89)] and four control and nine hyperoxic arteries of 100–200 μm [13.4 ± 2.6 (95% CI = 9.29–17.45) vs. 13.1 ± 0.9 (95% CI = 12.40–13.82)] (As shown in the drawing, thickness is expressed in micrometers in all instances).