Table 4.

Lowest percentage divergence thresholds required to generate a single OTU when clustering data for the 24 populations in the Tagged Populations Community. The number of individuals included within each population is indicated before the species name. For example, “5 x” indicates that five individuals were present. Note that >10% indicates that multiple OTUs were still generated even when applying a 10% divergence threshold.

	Percentage identity required to generate a single OTU
	Singletons excluded	Singletons included
5 × Corbicula fluminea	8	8
10 × Corbicula fluminea	3	8
30 × Corbicula fluminea	9	9
3 × Neotrypaea californiensis	2	3
5 × Balanus crenatus	4	6
10 × Balanus crenatus	4	4
17 × Balanus spp.	3	3
5 × Crangonyx	1	2
10 × Hyalella clade 8	5	6
5 × Daphnia mendotae	2	>10
10 × Daphnia pulex	2	3
31 × Daphnia pulex	2	3
5 × Leptodiaptomus minutus	4	4
9 × Leptodiaptomus sicilis	5	5
30 × Leptodiaptomus minutus	3	10
5 × Diacyclops thomasi	1	2
8 × Diacyclops thomasi	2	3
27 × Diacyclops thomasi	2	3
5 × Leptodora kindtii	3	4
10 × Leptodora kindtii	2	>10
28 × Leptodora kindtii	5	5
5 × Limnoperna fortunei	2	5
10 × Limnoperna fortunei	6	>10
30 × Limnoperna fortunei	2	4