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. 2004 Aug;78(15):8172–8182. doi: 10.1128/JVI.78.15.8172-8182.2004

FIG. 3.

FIG. 3.

NoV 3CLpro specifically inhibits poly(A)-dependent translation, and translation is restored by the addition of PABP. (A) Effect of NoV 3CLpro on translation efficiency of polyadenylated mRNA. Graphic representation of luciferase activity generated (relative light units [RLU] set to 100% of control reactions) using capped and polyadenylated luciferase RNA (left panel) or nonpolyadenylated luciferase RNA (right panel) in HeLa translation extracts incubated with increasing concentrations of NoV r3CLpro. Data represent the means ± standard deviations of four individual experiments. (B) Addition of exogenous PABP restores translation efficiency of polyadenylated mRNA in the presence of NoV 3CLpro. Comparison of polyadenylated (left panel) or nonpolyadenylated (right panel) luciferase RNA translation in HeLa translation extracts pretreated with buffer or 10 ng of NoV r3CLpro/μl and supplemented with His-PABP. Black bars represent the percent translation (light units relative to buffer control) in 3CLpro-treated lysates. Gray bars indicate luciferase translation levels after addition of His-PABP. Data represent the means ± standard deviations of three individual experiments.