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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: J Proteomics. 2015 May 14;125:104–111. doi: 10.1016/j.jprot.2015.04.033

Fig. 3. Confirmatory immunoblotting of select CrkL-SH3 binding proteins identified from E16.5 murine brain and liver.

Fig. 3

(A) Immunoblots of whole cell (tissue) extracts (WCE) and GST-CrkL-SH3 pulldowns using antibodies that recognize the indicated proteins. Approximate molecular weights in kDa are indicated at left. The Ponceau stain of the GST-CrkL-SH3 levels on the membrane prior to the blocking step is representative of all experiments. (B) Thirty micrograms of each tissue extract used in the pulldowns was separated by SDS-PAGE and the gel was stained with coommassie blue.