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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: J Proteomics. 2015 May 14;125:104–111. doi: 10.1016/j.jprot.2015.04.033

Fig. 4. C3G protein species variants of distinct molecular weights are variably enriched in embryonic murine brain and liver extracts and in CrkL-SH3 pulldowns from the same.

Fig. 4

(A) Immunoblotting using a polyclonal anti-C3G antibody (raised with an immunogen to approximately the most N-terminal 300 amino acids) was used to blot the whole cell (tissue) extracts (WCE) and GST-CrkL-SH3 pulldowns from embryonic murine brain and liver. Indicated are both the approximate molecular weights in kDa and the gel region used for the quantitative mass spectrometry. (B) Quantitative mass spectrometry exemplified by a mass spectrum of the indicated light and heavy precursor ion pair of the indicated peptide from C3G identified from gel region three. (C) Quantitative mass spectrometry results of all tryptic C3G peptides in biological replicate number two from the indicated gel regions that identified two or more C3G peptides. Quantifications are the Log2 ratio of the heavy/light (liver/brain) relative abundances from peptides in the CrkL-SH3 pulldowns. Box-and-Whisker plots for the peptides in each gel region are provided with means that are connected with a dotted red line. One way ANOVA analysis using an each pair Students-t test was performed and the statistical significance (*) is indicted between means of quantified C3G peptides in distinct sets of gel regions (p < 0.05).