(A, B) WT mice (n=5/group) were given FTY720. One day later untreated mice or FTY720-treated mice were injected with PBS or AMD3100. At 2.5 h p.i. the mice were bled and sacrificed for enumeration of (A) WBC, ANC, ALC and AMC and (B) specific leukocyte populations CD3+ T cells, B220+ B cells, CD4+ T cells and CD8+ T cells in the blood (top) and lymph nodes (bottom) by flow cytometry. Cells were gated on lymphocytes (supporting information 1). Results shown are from a single experiment representative of two independent experiments. Data are plotted for individual mice and summarized as the mean ± SEM. *p<0.05, **p<0.01, ***p<0.005, ****p<0.0001, two-tailed unpaired parametric t-test.