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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Bioorg Med Chem. 2015 Apr 16;23(13):3542–3551. doi: 10.1016/j.bmc.2015.04.022

Figure 3.

Figure 3

Induction of double-strand DNA breaks in K562 cells by mono- and bis-epipodophyllotoxin compounds as indicated by formation of γH2AX. K562 cells were treated with 25 μM of the compounds indicated for 4 h in growth medium, lysed and subjected to SDS-PAGE electrophoresis and Western blotting. The blots were probed with antibodies to γH2AX and with GAPDH (glyceraldehyde 3-phosphate dehydrogenase) as a loading control and a chemiluminescent-inducing horseradish peroxidase-conjugated secondary antibody.