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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: Am J Gastroenterol. 2015 Apr 21;110(6):899–908. doi: 10.1038/ajg.2015.97

Figure 8.

Figure 8

Epitope neutralization by lasB. Mixed gliadins (250 μg/ml) were incubated with 2.5 μg/ml lasB isolated in our laboratory. The mixture was incubated for 0, 0.5, and 2 h in 50 mM Tris-HCl (pH 8.0) buffer supplemented with 2.5 mM CaCl . Aliquotes were analyzed by SDS–PAGE (a) and the same aliquots were tested for epitope survival in R5 and G12 ELISA assays (c and d). In b, the hydrolysis of the FRET substrate QPQLPY (pep) (final 100 μM) by lasB (0.65 μg/ml) is shown. After 20 min incubation, the fluorescence had reached the maximum value that could still be read experimentally, indicating rapid substrate hydrolysis.